Posted on Sep 05, 2011
Session: Oral Communication 1 - Tuesday 20th – 2:00-2:15
Abstract ID# 3809
Chronic monitoring of brain tissue oxygen in freely moving rats from a fully implanted telemetry system.
Presenter: Simon Malpas
The ability to monitor tissue oxygen concentration in a specific region of the brain in a freely moving animal could provide new information currently difficult to obtain using alternative technologies.
We have developed a fully implantable telemetry system for the continuous and chronic recording of brain tissue oxygen (PO2,BR) in conscious animals. A telemetry system with a sampling rate of 2 kHz was combined with a miniaturized potentiostat to amperiometrically detect oxygen concentration with carbon paste electrodes (CPE). Wireless power was employed to inductively recharge the telemeter battery transcutaneously for potential lifetime monitoring.
Rats were implanted with the telemeter body in the peritoneal cavity and electrodes stereotaxically implanted into the brain (striatum or brainstem). Whilst animals were living in their home cages the sensitivity to changes in oxygen was validated by repeatedly altering the inspired oxygen (10%, 100%, respectively) or a pharmacological stimulus (carbonic anhydrase inhibitor: acetazolamide 50 mg/kg IP). Basal level of brain tissue PO2 could be monitored for many weeks (>6 weeks) and showed good overall stability, reproducibility and good correlation to common movements such as grooming.
During hypoxia, PO2,BR decreased significantly by -51±2 % from baseline whereas it increased by 34±3 % during hyperoxia. Immediately following the systemic acetazolamide PO2,BR increased by 38±4 %.
We propose this new technology provides a robust method to measure changes in oxygen concentration in a specific area of the brain, allowing conscious and freely moving measurements. The ability to track long term changes with disease progression or drug treatment may be enabled.
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